Abstract

Preclinical studies play a crucial role in scientific projects and the transition to clinical trials. These studies require formulating a sound hypothesis, conducting a comprehensive literature review to define the study design, determining the number of animals in the experiment, obtaining statistical support for interpreting results, and conducting all of these processes within an ethical framework. The selection of appropriate control groups and the use of pilot studies are also important. Unplanned and flawed practices in the design and execution of these studies not only hinder the transition to clinical application but also lead to a waste of research resources. This review will provide general information about experimental designs and offer an overview of the planning stages of preclinical studies.